The Vicco fire chief told WYMT TV In Hazard, a train derailed at a track crossing Yellow Creek Road in Knott County.
As of 10:45 p.m. Wednesday, only one lane of the road was open, but officials were expected to close both lanes eventually.
The chief said crews are waiting on equipment coming from Nicholasville to put the train back on its tracks.
The chief attributed the derailment to a minor mistake.
The Kentucky Transportation Cabinet reports the road is open again.